![]() Having independent depth-cueing for surface (nearest-point) and interior (brightest-point) allows for more visualization possibilities. For both kinds, depth-cueing is turned off when set to zero (i.e.100% of intensity in back to 100% of intensity in front) and is on when set at 0 < n 100 (i.e.( 100 − n)% of intensity in back to 100% intensity in front). Interior Depth-Cueing works only on brightest-point projections. Surface Depth-Cueing works only on nearest-point projections and the nearest-point component of other projections with opacity turned on. The Fill With choice does not matter, but choose Ramp. To install FIJI, download the version appropriate for your operating system here. Although I usually refer to the program as ImageJ in the following instructions, I am assuming you are actually using the FIJI version of ImageJ and NOT the basic version. If you are not working with a 32bit image, make a dummy image with File/New/Image. basic version of ImageJ cannot do this without loading additional plugins. Two kinds of depth-cueing are available: Surface Depth-Cueing and Interior Depth-Cueing. Add a Legend to ImageJ LUT Images This hint follows advice from Bob Dougherty: It is made with Tools/Calibration bar. The trade-off for this increased realism is that data points shown in a depth-cued image no longer possess accurate densitometric values. Description: Motion deblur reduces the blurring caused by objects in quick motion during the image acquisition process. ![]() The depth-cueing parameters determine whether projected points originating near the viewer appear brighter, while points further away are dimmed linearly with distance. Installation: Download and extract the contents of file motiondeblur.zip and simply put 'MotionDeblur.jar' into any plugin directory or subdirectory. Thus, differences in color in the pseudo-colored image reflect differences in intensity of the object rather than differences in color of the specimen that has been imaged. Surface/Interior Depth-Cueing Depth cues can contribute to the three-dimensional quality of projection images by giving perspective to projected structures. A LUT is literally a predefined table of gray values with matching red, green and blue values so that shadows of gray are displayed as colorized pixels.
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